pCMV-Tag4A-MMP8 (P78S)
(Plasmid
#29546)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Tag4A
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMetalloproteinase protein 8
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Alt nameMMP8
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SpeciesH. sapiens (human)
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Insert Size (bp)1404
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MutationP78S
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Entrez GeneMMP8 (a.k.a. CLG1, HNC, MMP-8, PMNL-CL)
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Tag4A-MMP8 (P78S) was a gift from Yardena Samuels (Addgene plasmid # 29546 ; http://n2t.net/addgene:29546 ; RRID:Addgene_29546) -
For your References section:
Analysis of the matrix metalloproteinase family reveals that MMP8 is often mutated in melanoma. Palavalli LH, Prickett TD, Wunderlich JR, Wei X, Burrell AS, Porter-Gill P, Davis S, Wang C, Cronin JC, Agrawal NS, Lin JC, Westbroek W, Hoogstraten-Miller S, Molinolo AA, Fetsch P, Filie AC, O'Connell MP, Banister CE, Howard JD, Buckhaults P, Weeraratna AT, Brody LC, Rosenberg SA, Samuels Y. Nat Genet. 2009 May . 41(5):518-20. 10.1038/ng.340 PubMed 19330028