pcDNA3.1-ERBB4 (E452K)
(Plasmid
#29529)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERBB4
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Alt nameHER4
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SpeciesH. sapiens (human)
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Insert Size (bp)3927
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Mutationchanged glutamic acid 452 to lysine
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GenBank IDNM_005235
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Entrez GeneERBB4 (a.k.a. ALS19, HER4, p180erbB4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-ERBB4 (E452K) was a gift from Yardena Samuels (Addgene plasmid # 29529 ; http://n2t.net/addgene:29529 ; RRID:Addgene_29529) -
For your References section:
Analysis of the tyrosine kinome in melanoma reveals recurrent mutations in ERBB4. Prickett TD, Agrawal NS, Wei X, Yates KE, Lin JC, Wunderlich JR, Cronin JC, Cruz P, Rosenberg SA, Samuels Y. Nat Genet. 2009 Oct . 41(10):1127-32. 10.1038/ng.438 PubMed 19718025