pCool-mRbx1-His-hCul1-CTP
(Plasmid #29519)

• Depositing Lab: Ning Zheng
• Publication: Ning Zheng Lab Plasmids (unpublished)

Backbone

• Vector backbone: pCool
• Backbone size w/o insert (bp): 5136
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rbx1 CUL1
• Alt name: Rbx1, CUL1
• Species: H. sapiens (human), M. musculus (mouse)
• Entrez Gene: CUL1
• Entrez Gene: Rbx1 (a.k.a. 1500002P15Rik, ROC1)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • Thrombin Cut Site (N terminal on backbone)
  • His (before thrombin) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Supplemental Documents:
  • pCool
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry