pAL-hCul1-NTD
(Plasmid #29518)

• Depositing Lab: Ning Zheng
• Publication: Ning Zheng Lab Plasmids (unpublished)

Backbone

• Vector backbone: pAL
• Backbone size w/o insert (bp): 4401
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CUL1
• Species: H. sapiens (human)
• Insert Size (bp): 1245
• Mutation: N-terminal 415 aa
• Entrez Gene: CUL1
• Tag / Fusion Protein:
  • Thrombin Cleavage Site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the 1st 12aa of Cul1 are missing. This region is disordered in the crystal structure.