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pFB-GTE-COI1
(Plasmid #29516)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-GTE
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COI1
  • Species
    A. thaliana (mustard weed)
  • Mutation
    Note that the first AA in TEV site is R, not E
  • Entrez Gene
    COI1 (a.k.a. AT2G39940, CORONATINE INSENSITIVE 1, T28M21.10, T28M21_10)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-GTE-COI1 was a gift from Ning Zheng (Addgene plasmid # 29516 ; http://n2t.net/addgene:29516 ; RRID:Addgene_29516)

  • For your References section:

    Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR, Kobayashi Y, Hsu FF, Sharon M, Browse J, He SY, Rizo J, Howe GA, Zheng N. Nature. 2010 Nov 18. 468(7322):400-5. 10.1038/nature09430 PubMed 20927106
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