Skip to main content
Addgene

pJ601
(Plasmid #29509)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29509 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pETBlue 1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4457
  • Vector type
    Bacterial Expression
  • Selectable markers
    Chloramphenicol for pLacI

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    annexin VI
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of the initiator Met at position 1.
  • GenBank ID
    NM_001155
  • Entrez Gene
    ANXA6 (a.k.a. ANX6, CBP68, CPB-II, p68, p70)
  • Tag / Fusion Protein
    • (Met)-Ala-Gly-Gly-Cys-Gly-His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RV (destroyed during cloning)
  • 3′ cloning site Eco RV (destroyed during cloning)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer pETBlueDOWN primer #70603-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ601 was a gift from Jonathan Tait (Addgene plasmid # 29509 ; http://n2t.net/addgene:29509 ; RRID:Addgene_29509)
Related items:
Commonly requested with: