pBacPAK8-His-VHL-Flag
(Plasmid
#29497)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5500
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVon Hippel-Lindau
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Alt nameVHL
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SpeciesH. sapiens (human)
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Entrez GeneVHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site don't know (unknown if destroyed)
- 3′ cloning site don't know (unknown if destroyed)
- 5′ sequencing primer Bac1 Primer
- 3′ sequencing primer Bac2 Primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBacPAK8-His-VHL-Flag was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29497 ; http://n2t.net/addgene:29497 ; RRID:Addgene_29497) -
For your References section:
Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5. 10.1073/pnas.190332597 PubMed 10973499