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pJ143
(Plasmid #29490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETBlue 1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4457
  • Vector type
    Bacterial Expression
  • Selectable markers
    Chloramphenicol for pLacI

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    annexin V-128
  • Alt name
    Annexin A5-128
  • Alt name
    Annexin V
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    980
  • Mutation
    Deletion of the initiator Met at position 1 of wild-type annexin V and a point mutation changing Cys 316 to Ser. Glu72Gln (caa), Asp144Asn (aac), and Asp303Asn (aat)
  • GenBank ID
    NM_001154
  • Entrez Gene
    ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
  • Tag / Fusion Protein
    • (Met)-Ala-Gly-Gly-Cys-Gly-His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RV (destroyed during cloning)
  • 3′ cloning site Eco RV (destroyed during cloning)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer pETBlueDOWN primer #70603-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ143 was a gift from Jonathan Tait (Addgene plasmid # 29490 ; http://n2t.net/addgene:29490 ; RRID:Addgene_29490)

  • For your References section:

    Essential role of B-helix calcium binding sites in annexin V-membrane binding. Jin M, Smith C, Hsieh HY, Gibson DF, Tait JF. J Biol Chem 2004; 279:40351-57. 10.1074/jbc.M405846200 PubMed 15280367
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