pJ131
(Plasmid #29485)

• Depositing Lab: Jonathan Tait
• Publication: Jin et al J Biol Chem 2004; 279:40351-57.

Backbone

• Vector backbone: pETBlue 1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4457
• Vector type: Bacterial Expression
• Selectable markers: Chloramphenicol for pLacI

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: annexin V-128
• Alt name: Annexin A5-128
• Alt name: Annexin V
• Species: H. sapiens (human)
• Insert Size (bp): 980
• Mutation: Deletion of the initiator Met at position 1 of wild-type annexin V and a point mutation changing Cys 316 to Ser. Asp303Asn (aat)
• GenBank ID: NM_001154
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
• Tag / Fusion Protein:
  • (Met)-Ala-Gly-Gly-Cys-Gly-His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Eco RV (destroyed during cloning)
• 3′ cloning site: Eco RV (destroyed during cloning)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: pETBlueDOWN primer #70603-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry