pMX155
(Plasmid
#29481)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4706
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKif2A-alpha
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SpeciesM. musculus (mouse)
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Insert Size (bp)2152
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MutationE140K
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GenBank IDD12644
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Entrez GeneKif2a (a.k.a. C530030B14Rik, Kif2, Kns2, M-kinesin)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor believes the mutation in Kif2a is the results of either errors in database sequences or polymorphisms.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX155 was a gift from Linda Wordeman (Addgene plasmid # 29481 ; http://n2t.net/addgene:29481 ; RRID:Addgene_29481) -
For your References section:
MCAK associates with the tips of polymerizing microtubules. Moore AT, Rankin KE, von Dassow G, Peris L, Wagenbach M, Ovechkina Y, Andrieux A, Job D, Wordeman L. J Cell Biol. 2005 May 9. 169(3):391-7. 10.1083/jcb.200411089 PubMed 15883193