GFP-Tog
(Plasmid #29480)

• Depositing Lab: Linda Wordeman
• Publication: Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4714
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ch-TOG
• Species: H. sapiens (human)
• Insert Size (bp): 5950
• Mutation: K19R, E118G, G254R, K1834R
• Entrez Gene: CKAP5 (a.k.a. CHTOG, MSPS, TOG, TOGp, ch-TOG)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Christian Larroque INSERM, Montpellier, France
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor believes the mutations in Tog are the results of either errors in database sequences or polymorphisms.