Skip to main content
Addgene

pMX230
(Plasmid #29479)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4707
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2052
  • GenBank ID
    BC033802
  • Entrez Gene
    KIF2B
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer CMV_fwd_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX230 was a gift from Linda Wordeman (Addgene plasmid # 29479 ; http://n2t.net/addgene:29479 ; RRID:Addgene_29479)

  • For your References section:

    The kinesin-13 proteins Kif2a, Kif2b, and Kif2c/MCAK have distinct roles during mitosis in human cells. Manning AL, Ganem NJ, Bakhoum SF, Wagenbach M, Wordeman L, Compton DA. Mol Biol Cell. 2007 Aug . 18(8):2970-9. 10.1091/mbc.e07-02-0110 PubMed 17538014
Related items:
Commonly requested with: