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Addgene

pJAK2.D
(Plasmid #29465)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29465 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMV261
  • Backbone size (bp) 4492
  • Vector type
    Bacterial Expression ; Mycobacteria expression
  • Promoter Acetamide promoter
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdb survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Replicative mycobacterial destination vector for recombination cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAK2.D was a gift from Zakaria Hmama (Addgene plasmid # 29465 ; http://n2t.net/addgene:29465 ; RRID:Addgene_29465)
  • For your References section:

    A broad-range of recombination cloning vectors in mycobacteria. Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z. Plasmid. 2009 Nov . 62(3):158-65. 10.1016/j.plasmid.2009.07.003 PubMed 19647016