pJAK2.B
(Plasmid
#29463)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMV261
- Backbone size (bp) 4492
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Vector typeBacterial Expression ; Mycobacteria expression
- Promoter Hsp60
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Tag
/ Fusion Protein
- M. tuberculosis FbpB signal sequence (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdb survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Replicative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJAK2.B was a gift from Zakaria Hmama (Addgene plasmid # 29463 ; http://n2t.net/addgene:29463 ; RRID:Addgene_29463) -
For your References section:
A broad-range of recombination cloning vectors in mycobacteria. Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z. Plasmid. 2009 Nov . 62(3):158-65. 10.1016/j.plasmid.2009.07.003 PubMed 19647016
Map uploaded by the depositor.
