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Addgene

SEMA3E (mouse) pBLUE
(Plasmid #29453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBLUESCRIPT (KS)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    pBlue for ISH

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sema3E (mouse)
  • Alt name
    Semaphorin 3E
  • Alt name
    Collapsin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Mutation
    1400bp fragment for ISH
  • GenBank ID
    NM_011348
  • Entrez Gene
    Sema3e (a.k.a. 6430702L12, AA408817, Semah, mKIAA0331)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEMA3E (mouse) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29453 ; http://n2t.net/addgene:29453 ; RRID:Addgene_29453)
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