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Addgene

AP-SEMA3A (chick)
(Plasmid #29448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-SEMA3A (chick)
  • Alt name
    AP-Collapsin1
  • Alt name
    Collapsin1
  • Alt name
    Semaphorin 3A
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3800
  • GenBank ID
    NM_204977
  • Entrez Gene
    SEMA3A (a.k.a. COLL-1, semaphorin-3A)
  • Tag / Fusion Protein
    • AP tagged (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer TTC GGC TTC TGG CGT GTG ACC
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AP-SEMA3A (chick) was a gift from Jonathan Raper (Addgene plasmid # 29448 ; http://n2t.net/addgene:29448 ; RRID:Addgene_29448)

  • For your References section:

    Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ. Feiner L, Koppel AM, Kobayashi H, Raper JA. Neuron. 1997 Sep . 19(3):539-45. 10.1016/S0896-6273(00)80370-0 PubMed 9331347
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