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Addgene

pMAX deltaN-ERG
(Plasmid #29447)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAX-DEST
  • Backbone manufacturer
    Amaxa
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    Deletion of first 33 aa of ERG
  • Entrez Gene
    ERG (a.k.a. LMPHM14, erg-3, p55)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR fragment amplified using IMAGE clone 6052140. Cloned into pCR8/GW/TOPO and then moved to pMAX-DEST using Gateway.

For transient expression of human deltaN-ERG. Has endogenous stop codon (no V5).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAX deltaN-ERG was a gift from Valeri Vasioukhin (Addgene plasmid # 29447 ; http://n2t.net/addgene:29447 ; RRID:Addgene_29447)

  • For your References section:

    A causal role for ERG in neoplastic transformation of prostate epithelium. Klezovitch O, Risk M, Coleman I, Lucas JM, Null M, True LD, Nelson PS, Vasioukhin V. Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):2105-10. 10.1073/pnas.0711711105 PubMed 18245377
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