pcDNA5FRT/Flag-hINO80N2 (1-406)
(Plasmid
#29442)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5-FRT
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5070
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINO80
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Alt namepcDNA5FRT/Flag-hINO80N2
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SpeciesH. sapiens (human)
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Insert Size (bp)1218
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MutationINO80 1-406 (a.a.)
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GenBank IDNP_060023
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Entrez GeneINO80 (a.k.a. INO80A, INOC1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5FRT/Flag-hINO80N2 (1-406) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29442 ; http://n2t.net/addgene:29442 ; RRID:Addgene_29442) -
For your References section:
Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2011 Feb 8. ():. 10.1074/jbc.M111.222505 PubMed 21303910