pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)
(Plasmid
#29440)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5-FRT
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5070
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINO80
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Alt namepcDNA5FRT/FLAG-Ino80 dN
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SpeciesH. sapiens (human)
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Insert Size (bp)3873
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MutationINO80 267-1556 (a.a.)
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GenBank IDNP_060023
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Entrez GeneINO80 (a.k.a. INO80A, INOC1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal/XhoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5FRT/FLAG-Ino80 ΔN (267-1556) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29440 ; http://n2t.net/addgene:29440 ; RRID:Addgene_29440) -
For your References section:
Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2011 Feb 8. ():. 10.1074/jbc.M111.222505 PubMed 21303910