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pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)
(Plasmid #29438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INO80
  • Alt name
    pcDNA5FRT/FLAG-Ino80 dN-EQ
  • Species
    H. sapiens (human)
  • Mutation
    INO80 267-1556 (a.a.) E653Q
  • GenBank ID
    NP_060023
  • Entrez Gene
    INO80 (a.k.a. INO80A, INOC1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI / XhoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29438 ; http://n2t.net/addgene:29438 ; RRID:Addgene_29438)

  • For your References section:

    Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2011 Feb 8. ():. 10.1074/jbc.M111.222505 PubMed 21303910
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