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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4756
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDJ1
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SpeciesM. musculus (mouse)
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Entrez GenePark7 (a.k.a. DJ-1, Dj1)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-mouse-DJ1-HA was a gift from Mark Cookson (Addgene plasmid # 29396 ; http://n2t.net/addgene:29396 ; RRID:Addgene_29396) -
For your References section:
Identification of the epitope of a monoclonal antibody to DJ-1. Miller DW, Wilson CR, Kaleem MA, Blackinton J, Cookson MR. Neurosci Lett. 2005 Feb 21. 374(3):203-6. 10.1016/j.neulet.2004.10.088 PubMed 15663963