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pTH355-SUP45-T55A
(Plasmid #29377)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 5976
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUP45 gene encoding translation release factor 1 from S. cerevisiae
  • Alt name
    eRF1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2984
  • Mutation
    T55A mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon.
  • GenBank ID
    NM_001178491
  • Entrez Gene
    SUP45 (a.k.a. YBR143C, SAL4, SUP1, SUP47)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH355-SUP45-T55A was a gift from Tobias von der Haar (Addgene plasmid # 29377 ; http://n2t.net/addgene:29377 ; RRID:Addgene_29377)

  • For your References section:

    Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast. Merritt GH, Naemi WR, Mugnier P, Webb HM, Tuite MF, von der Haar T. Nucleic Acids Res. 2010 Sep . 38(16):5479-92. 10.1093/nar/gkq338 PubMed 20444877
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