pGEX-5X-1-DJ1-M26I
(Plasmid
#29353)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-5X-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDJ1
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SpeciesH. sapiens (human)
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MutationMet26Ile
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GenBank IDAF021819
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Entrez GenePARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-5X-1-DJ1-M26I was a gift from Mark Cookson (Addgene plasmid # 29353 ; http://n2t.net/addgene:29353 ; RRID:Addgene_29353) -
For your References section:
Effects of DJ-1 mutations and polymorphisms on protein stability and subcellular localization. Blackinton J, Ahmad R, Miller DW, van der Brug MP, Canet-Aviles RM, Hague SM, Kaleem M, Cookson MR. Brain Res Mol Brain Res. 2005 Mar 24. 134(1):76-83. 10.1016/j.molbrainres.2004.09.004 PubMed 15790532