pGW1-Myc-DJ1-WT
(Plasmid #29347)

• Depositing Lab: Mark Cookson
• Publication: Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-95.

Backbone

• Vector backbone: pGW1myc2b
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DJ1
• Species: H. sapiens (human)
• GenBank ID: NP_009193.2.
• Entrez Gene: PARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: M13_puc_Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry