pcDNA3.1/GS-DJ1-E64D-V5
(Plasmid #29344)

• Depositing Lab: Mark Cookson
• Publication: Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-95.

Backbone

• Vector backbone: pcDNA3.1/GS
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DJ1
• Species: H. sapiens (human)
• Mutation: Glu64Asp
• GenBank ID: AF021819
• Entrez Gene: PARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)
• Tags / Fusion Proteins:
  • V5 epitope (C terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry