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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSVL
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4896
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDimer of HDV genome with 2 nucleotide deletion in delta antigen
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SpeciesHepatitis Delta Virus
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Insert Size (bp)3346
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MutationContains a dimer of the complete HDV genome except for a deletion of 2bp at 1434 to 1435 (within the delta antigen)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSVL(D2m) was a gift from John Taylor (Addgene plasmid # 29336 ; http://n2t.net/addgene:29336 ; RRID:Addgene_29336) -
For your References section:
Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. Kuo MY, Chao M, Taylor J. J Virol. 1989 May . 63(5):1945-50. PubMed 2649689
