pEMS1503
(Plasmid #29299)

• Purpose: Homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ reporter.
• Depositing Lab: Elizabeth Simpson
• Publication: de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:5.

Backbone

• Vector backbone: pEMS1313
• Backbone size w/o insert (bp): 18843
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple32
• Alt name: CLDN5
• Alt name: Claudin 5
• Species: H. sapiens (human)
• Insert Size (bp): 1670
• Entrez Gene: CLDN5 (a.k.a. AWAL, BEC1, CPETRL1, TMDVCF, TMVCF)
• Tag / Fusion Protein:
  • Intron-LacZ-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA