pEMS1613
(Plasmid #29278)

• Purpose: Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
• Depositing Lab: Elizabeth Simpson
• Publication: Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.

Backbone

• Vector backbone: pEMS1313
• Backbone size w/o insert (bp): 18843
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple141
• Alt name: NR2E1
• Alt name: Orphan nuclear receptor NR2E1
• Species: H. sapiens (human)
• Insert Size (bp): 4231
• Entrez Gene: NR2E1 (a.k.a. TLL, TLX, XTLL)
• Tag / Fusion Protein:
  • Intron-LacZ-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a 6 nucleotide deletion at bp# 139-144 and a 6 nucleotide deletion at bp# 4376-7381. These deletions are not known to affect the function of the plasmid.