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pEMS1593
(Plasmid #29270)

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Item Catalog # Description Quantity Price (USD)
Plasmid 29270 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pEMS1313
  • Backbone size w/o insert (bp) 18843
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple122
  • Alt name
    ICMT
  • Alt name
    isoprenylcysteine carboxyl methyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2222
  • Entrez Gene
    ICMT (a.k.a. HSTE14, MST098, MSTP098, PCCMT, PCMT, PPMT)
  • Tag / Fusion Protein
    • Intron-LacZ-NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1593 was a gift from Elizabeth Simpson (Addgene plasmid # 29270 ; http://n2t.net/addgene:29270 ; RRID:Addgene_29270)

  • For your References section:

    Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. de Leeuw CN, Dyka FM, Boye SL, Laprise S, Zhou M, Chou AY, Borretta L, McInerny SC, Banks KG, Portales-Casamar E, Swanson MI, D'Souza CA, Boye SE, Jones SJ, Holt RA, Goldowitz D, Hauswirth WW, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2014 Jan 8;1:5. 10.1038/mtm.2013.5 PubMed 24761428
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