pEMS1485
(Plasmid #29236)

• Purpose: Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).
• Depositing Lab: Elizabeth Simpson
• Publication: de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:5.

Backbone

• Vector backbone: pEMS1313
• Backbone size w/o insert (bp): 18843
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple15
• Alt name: C8ORF46
• Alt name: MGC33510
• Species: H. sapiens (human)
• Insert Size (bp): 3536
• Entrez Gene: C8orf46 (a.k.a. MGC33510)
• Tag / Fusion Protein:
  • Intron-LacZ-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA