pEMS1387
(Plasmid #29186)

• Purpose: Expression of the eGFP reporter gene was not detected in the brain and eye.
• Depositing Lab: Elizabeth Simpson
• Publication: Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.

Backbone

• Vector backbone: pEMS1306
• Backbone size w/o insert (bp): 16454
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple118
• Alt name: HTR1A
• Alt name: 5-hydroxytryptamine 1A receptor
• Species: H. sapiens (human)
• Insert Size (bp): 3102
• Entrez Gene: HTR1A (a.k.a. 5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD)
• Tag / Fusion Protein:
  • EGFP-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvrII (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pEMS1387 GenBank file
  • pEMS1387 PCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry