pEMS1210
(Plasmid #29107)

• Purpose: Expression of the eGFP reporter gene was not detected in the brain and eye.
• Depositing Lab: Elizabeth Simpson
• Publication: de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:5.

Backbone

• Vector backbone: pEMS1307
• Backbone size w/o insert (bp): 16482
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple238
• Alt name: TRH
• Alt name: thyrotropin releasing hormone
• Species: H. sapiens (human)
• Insert Size (bp): 3215
• Entrez Gene: TRH (a.k.a. Pro-TRH, TRF)
• Tag / Fusion Protein:
  • EGFP-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pEMS1210 GenBank file
  • pEMS1210 PCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry