pEMS1182
(Plasmid #29094)

• Purpose: Expression of the eGFP reporter gene was not detected in the brain and eye.
• Depositing Lab: Elizabeth Simpson
• Publication: Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.

Backbone

• Vector backbone: pEMS1306
• Backbone size w/o insert (bp): 16454
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple219
• Alt name: TAC3
• Alt name: tachykinin 2
• Species: H. sapiens (human)
• Insert Size (bp): 1802
• Entrez Gene: TAC3 (a.k.a. HH10, NKB, NKNB, PRO1155, ZNEUROK1)
• Tag / Fusion Protein:
  • EGFP-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pEMS1182 GenBank file
  • pEMS1182 PCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a 20 nucleotide deletion at bp# 116-136. This deletion is not known to affect the function of the plasmid.