pEMS1166
(Plasmid #29081)

• Purpose: Expression of the eGFP reporter gene was not detected in the brain and eye.
• Depositing Lab: Elizabeth Simpson
• Publication: Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.

Backbone

• Vector backbone: pEMS1306
• Backbone size w/o insert (bp): 16454
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple61
• Alt name: DRD1
• Alt name: dopamine receptor D1
• Species: H. sapiens (human)
• Insert Size (bp): 3944
• Entrez Gene: DRD1 (a.k.a. D1R, DADR, DRD1A)
• Tag / Fusion Protein:
  • EGFP-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pEMS1166 GenBank file
  • pEMS1166 PCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a deletion from bp#137-149. This difference is not known to affect the function of the plasmid.