pEMS1136
(Plasmid #29056)

• Purpose: Expression of the eGFP reporter gene was not detected in the brain and eye.
• Depositing Lab: Elizabeth Simpson
• Publication: Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.

Backbone

• Vector backbone: pEMS1307
• Backbone size w/o insert (bp): 16482
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple198
• Alt name: SLC6A4
• Alt name: solute carrier family 6, member 4
• Species: H. sapiens (human)
• Insert Size (bp): 2826
• Entrez Gene: SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
• Tag / Fusion Protein:
  • EGFP-NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pEMS1136 GenBank file
  • pEMS1136 PCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry