Skip to main content
Addgene

pEMS1099
(Plasmid #29030)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEMS1302
  • Backbone size w/o insert (bp) 17451
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple231
  • Alt name
    TNNT1
  • Alt name
    Troponin T, slow skeletal muscle
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1258
  • Entrez Gene
    TNNT1 (a.k.a. ANM, NEM5, STNT, TNT, TNTS)
  • Tag / Fusion Protein
    • EGFP-Cre-NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)

Please note that Addgene's sequencing results identified a deletion/mismatch region at bp#108-136 and two single nucelotide mismatches at bp#535 & 655 when compared to the full sequence information provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1099 was a gift from Elizabeth Simpson (Addgene plasmid # 29030 ; http://n2t.net/addgene:29030 ; RRID:Addgene_29030)

  • For your References section:

    A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. 10.1073/pnas.1009158107 PubMed 20807748
Related items: