pAAV-FLEX-GFP
(Plasmid #28304)

• Depositing Lab: Edward Boyden
• Publication: Accessory plasmids for optogenetics (unpublished)

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Backbone

• Vector backbone: AAV-FLEX
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 5047
• Vector type: Mammalian Expression, AAV, Cre/Lox ; Adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: Green fluorescent protein
• Species: A. victoria
• Insert Size (bp): 720
• Mutation: N/A
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: cttctggcgtgtgaccgg

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.