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Addgene

DCX 3UTRm3hp
(Plasmid #28302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mU6pro
  • Backbone manufacturer
    University of Michigan
  • Backbone size w/o insert (bp) 4143
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha subcloning efficiency cells at 37deg C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Doublecortin 3' Untranslated region mutated
  • Alt name
    DCX 3'UTRm3 hairpin
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    53
  • Mutation
    3 base pair mutation in DCX 3'UTR region

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbs 1 (unknown if destroyed)
  • 3′ cloning site Xba 1 (unknown if destroyed)
  • 5′ sequencing primer M13R2 reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DCX 3’UTRm3 mutation hairpin siRNA sequence:
5’-GCUCAAGUCACGAAGAAGGCUAUAGACACAAUAGCCUUCUUCGUGACUUGAGC-3’

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DCX 3UTRm3hp was a gift from Joseph Loturco (Addgene plasmid # 28302 ; http://n2t.net/addgene:28302 ; RRID:Addgene_28302)

  • For your References section:

    RNAi reveals doublecortin is required for radial migration in rat neocortex. Bai J, Ramos RL, Ackman JB, Thomas AM, Lee RV, LoTurco JJ. Nat Neurosci. 2003 Dec . 6(12):1277-83. 10.1038/nn1153 PubMed 14625554
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