pCMV-NHERF1-EB
(Plasmid
#28297)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-2-FLAG
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNHERF1
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SpeciesM. musculus (mouse)
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MutationContains only the EB domain aa 262-355 (deletion of the PDZ1 and PDZ2)
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Entrez GeneNherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-NHERF1-EB was a gift from Maria-Magdalena Georgescu (Addgene plasmid # 28297 ; http://n2t.net/addgene:28297 ; RRID:Addgene_28297) -
For your References section:
NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM. Mol Cell Biol. 2007 Apr . 27(7):2527-37. 10.1128/MCB.01372-06 PubMed 17242191