pCMV-NHERF1-PDZ1-2
(Plasmid #28294)

• Depositing Lab: Maria-Magdalena Georgescu
• Publication: Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Backbone

• Vector backbone: pCMV-2-FLAG
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NHERF1
• Species: H. sapiens (human)
• Mutation: Contains aa 1-298 (deletion of the EB domain aa 299-358)
• Entrez Gene: NHERF1 (a.k.a. EBP50, NHE-RF, NHERF, NHERF-1, NPHLOP2, SLC9A3R1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a mutation (N268S) in the NHERF1 sequence, which corresponds to the mouse NHERF1 sequence at this position. The depositing scientist states that the mutation does not affect NHERF1 function.