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Addgene

ch-Tog
(Plasmid #28290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28290 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Script
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ch-TOG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6449
  • Mutation
    K19R, E118G
  • GenBank ID
    X92474
  • Entrez Gene
    CKAP5 (a.k.a. CHTOG, MSPS, TOG, TOGp, ch-TOG)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Lynne Cassimeris Lehigh University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor believes the mutations in Tog are the results of either errors in database sequences or polymorphisms.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ch-Tog was a gift from Linda Wordeman (Addgene plasmid # 28290 ; http://n2t.net/addgene:28290 ; RRID:Addgene_28290)

  • For your References section:

    MCAK associates with the tips of polymerizing microtubules. Moore AT, Rankin KE, von Dassow G, Peris L, Wagenbach M, Ovechkina Y, Andrieux A, Job D, Wordeman L. J Cell Biol. 2005 May 9. 169(3):391-7. 10.1083/jcb.200411089 PubMed 15883193
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