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Addgene

PP-HSP90 (3Q5J)
(Plasmid #28281)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28281 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET15-MHL (Addgene plasmid 26092)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7737
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PP-HSP90
  • Alt name
    PDB: 3Q5J, 3Q5L, 3Q5K, 3U67
  • Alt name
    heat shock protein 90
  • Species
    Leishmania major
  • Insert Size (bp)
    639
  • Mutation
    N-terminal domain
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site LIC (unknown if destroyed)
  • 3′ cloning site LIC (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PP-HSP90 (3Q5J) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28281 ; http://n2t.net/addgene:28281 ; RRID:Addgene_28281)
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