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Addgene

PHF20 (3Q1J)
(Plasmid #28278)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28278 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PHF20
  • Alt name
    PDB: 3Q1J
  • Alt name
    PHD finger protein 20
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    240
  • Mutation
    residues 1-81.
  • Entrez Gene
    PHF20 (a.k.a. C20orf104, GLEA2, HCA58, NZF, TDRD20A, TZP)
  • Tag / Fusion Protein
    • His with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PHF20 (3Q1J) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28278 ; http://n2t.net/addgene:28278 ; RRID:Addgene_28278)
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