Skip to main content
Addgene

TRIM54
(Plasmid #28277)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28277 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRIM54
  • Alt name
    PDB: 3Q1D
  • Alt name
    Tripartite motif-containing protein 54
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    186
  • Mutation
    B-Box domain, residues 122-168.
  • Entrez Gene
    TRIM54 (a.k.a. MURF, MURF-3, RNF30, muRF3)
  • Tag / Fusion Protein
    • His with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRIM54 was a gift from Cheryl Arrowsmith (Addgene plasmid # 28277 ; http://n2t.net/addgene:28277 ; RRID:Addgene_28277)
Related items:
Commonly requested with: