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Addgene

ARHGAP27A
(Plasmid #28276)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28276 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pNIC-CH (Addgene plasmid 26117)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7218
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ARHGAP27A
  • Alt name
    PDB: 3PP2
  • Alt name
    Rho GTPase activating protein 27
  • Alt name
    CAMGAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1197
  • Mutation
    contains PH and RhoGAP domains (residues 491-613).
  • GenBank ID
    BC101388
  • Entrez Gene
    ARHGAP27 (a.k.a. CAMGAP1, FLJ35572, FLJ43547, MGC120624)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ligation-Independent cloning (destroyed during cloning)
  • 3′ cloning site Ligation-Independent cloning (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ARHGAP27A was a gift from Cheryl Arrowsmith (Addgene plasmid # 28276 ; http://n2t.net/addgene:28276 ; RRID:Addgene_28276)
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