TDRD3 (3PMT)
(Plasmid
#28274)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 28274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MHL (Addgene plasmid 26096)
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7325
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTudor domain-containing protein 3
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Alt namePDB: 3PMT, 6V9T
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Alt nameTudor domain-containing protein 3
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SpeciesH. sapiens (human)
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Insert Size (bp)174
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Mutationcontains Tudor domain, residues 553-611.
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Entrez GeneTDRD3 (a.k.a. RP11-459E2.1, FLJ21007)
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Tag
/ Fusion Protein
- His with TEV cleavage (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ligation-independent cloning (unknown if destroyed)
- 3′ cloning site ligation-independent cloning (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDRD3 (3PMT) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28274 ; http://n2t.net/addgene:28274 ; RRID:Addgene_28274)