pEF-HA/Net1A
(Plasmid
#28262)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-HA
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNet1A
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Alt nameneuroepithelial cell transforming gene 1 transcript variant 2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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GenBank IDNM_001047159
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Entrez GeneNet1 (a.k.a. Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik)
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Tag
/ Fusion Protein
- 2x HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF-1a-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-HA/Net1A was a gift from Jeffrey Frost (Addgene plasmid # 28262 ; http://n2t.net/addgene:28262 ; RRID:Addgene_28262) -
For your References section:
Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. Carr HS, Cai C, Keinanen K, Frost JA. J Biol Chem. 2009 Sep 4. 284(36):24269-80. 10.1074/jbc.M109.029439 PubMed 19586902