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Addgene

pGEX-KG/Net1
(Plasmid #28260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5006
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Net1
  • Alt name
    neuroepithelial cell transforming gene 1 transcript variant 1
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_019671
  • Entrez Gene
    Net1 (a.k.a. Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains wt mNet1 (aa 1-595)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-KG/Net1 was a gift from Jeffrey Frost (Addgene plasmid # 28260 ; http://n2t.net/addgene:28260 ; RRID:Addgene_28260)

  • For your References section:

    Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. Carr HS, Cai C, Keinanen K, Frost JA. J Biol Chem. 2009 Sep 4. 284(36):24269-80. 10.1074/jbc.M109.029439 PubMed 19586902
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