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pET-sCS4-2T
(Plasmid #28250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET42+b
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid is provided in DH5a cells. BL21-CodonPlus (DE3)-RILP Competent Cells from Agilent are recommended for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single chain Cse4-Scm3-H4 with 2 Thrombin site
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    700
  • Mutation
    A thrombin cutting site between Cse4 and Scm3. Another one between Scm3 and H4
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 pro
  • 3′ sequencing primer T7 ter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-sCS4-2T was a gift from Yawen Bai (Addgene plasmid # 28250 ; http://n2t.net/addgene:28250 ; RRID:Addgene_28250)

  • For your References section:

    Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins LM, Xiao H, Tjandra N, Wu C, Bai Y. Nature. 2011 Apr 14. 472(7342):234-7. 10.1038/nature09854 PubMed 21412236
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