pEYFP-C1-NuMA
(Plasmid #28238)

• Purpose: Expresses human NuMA with N-terminal EYFP tag in mammalian cells
• Depositing Lab: Michael Mancini
• Publication: Mancini Lab Plasmids (unpublished)

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: XL10 gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1)
• Species: H. sapiens (human)
• Insert Size (bp): 6306
• GenBank ID:
• Entrez Gene: NUMA1 (a.k.a. NMP-22, NUMA)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Shuo Dong, Baylor College of Medicine
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NuMA insert contains E873K and L1575F mutations compared to best match reference sequence (NP_001273490.1).